通过磷-31核磁共振光谱法测定的脱氧核糖核酸A形式中磷酸二酯主链的几何结构。
Geometry of the phosphodiester backbone in the A form of deoxyribonucleic acid determined by phosphorus-31 nuclear magnetic resonance spectroscopy.
作者信息
Shindo H, Wooten J B, Zimmerman S B
出版信息
Biochemistry. 1981 Feb 17;20(4):745-50. doi: 10.1021/bi00507a013.
31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orientation of the phosphodiester group relative to the helical axis was determined to be beta = 70 degrees and gamma = 52 degrees. The 31P NMR spectra of poly(dA-dT) . poly(dA-dT) were in remarkable contrast to the 31P NMR spectra of the A form of natural DNA, which exhibited an unusual line shape. The origins of the abnormalities in the line shape for the A form of natural DNA are discussed in terms of phosphodiester orientations.
从聚（dA-dT）·聚（dA-dT）纤维获得了31P核磁共振谱，该纤维给出的X射线衍射图谱与天然脱氧核糖核酸（DNA）的A形式相似。线形分析表明，聚（dA-dT）·聚（dA-dT）的A形式具有单一均匀的主链构象；磷酸二酯基团相对于螺旋轴的取向确定为β = 70度和γ = 52度。聚（dA-dT）·聚（dA-dT）的31P核磁共振谱与天然DNA的A形式的31P核磁共振谱形成显著对比，后者呈现出异常的线形。根据磷酸二酯取向讨论了天然DNA的A形式线形异常的起源。
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