Benz R, Zimmermann U
Biochim Biophys Acta. 1981 Jan 8;640(1):169-78. doi: 10.1016/0005-2736(81)90542-3.
The resealing process of lipid bilayer membranes after reversible electrical breakdown was investigated using two voltage pulses switched on together. Electrical breakdown of the membranes was induced with a voltage pulse of high intensity and short duration. The time course of the change in membrane conductance after the application of the high (short) voltage pulse was measured with a longer voltage pulse of low amplitude. The decrease in membrane conductance during the resealing process could be fitted to a single exponential curve with a time constant of 10--2 micros in the temperature range between 2 and 20 degrees C. The activation energy for this exponential decay process was found to be about 50 kJ/mol, which might indicate a diffusion process. Above 25 degrees C the resealing process is controlled by two exponential processes. The data obtained for the time course of the resealing process can be explained in terms of pore formation in the membrane in response to the high electrical field strength. A radius of about 4 nm is calculated for the initial pore size. From the assumed exponential change of the pore area with progressive resealing time a diffusion constant of 10(-8) cm2/s for lateral lipid diffusion can be estimated.
使用同时开启的两个电压脉冲，研究了可逆电击穿后脂质双层膜的重新封闭过程。用高强度短持续时间的电压脉冲诱导膜的电击穿。施加高（短）电压脉冲后，用低幅度的较长电压脉冲测量膜电导变化的时间进程。在2至20摄氏度的温度范围内，重新封闭过程中膜电导的降低可拟合为时间常数为10 - 2微秒的单指数曲线。发现该指数衰减过程的活化能约为50 kJ/mol，这可能表明是一个扩散过程。高于25摄氏度时，重新封闭过程由两个指数过程控制。重新封闭过程时间进程所获得的数据可以根据膜响应高电场强度形成孔来解释。计算出初始孔径约为4 nm。根据假定的孔面积随重新封闭时间的指数变化，可以估计横向脂质扩散的扩散常数为10(-8) cm2/s。
