Hope M J, Cullis P R
Biochim Biophys Acta. 1981 Jan 8;640(1):82-90. doi: 10.1016/0005-2736(81)90533-2.
(1) A comparative study of the polymorphic phase behaviour of reconstituted erythrocyte phospholipase with 'fusogens' and chemically related 'non-fusogens' using 31P-NMR techniques has been made. We demonstrate that only fusogens can induce a conformational change in the membrane lipids from the bilayer of the hexagonal (HII) phase. (2) The tendency of erythrocyte (ghost) membranes to adopt nonbilayer structures is correlated with the extent of fusion in erythrocytes at various membrane concentrations of glycerol monooleate. Above a certain critical membrane concentration of glycerol monooleate, there is an increase in the tendency of ghost membranes to adopt the HII phase, which is paralleled by a marked increase in fusion processes in the intact erythrocytes. (3) These observations are consistent with a common mechanism of action for fusogenic lipids whereby inverted lipid structures such as long cylinders (HII configuration) or inverted micelles are induced, which serve as intermediates in the fusion event.
（1）利用³¹P-NMR技术对重组红细胞磷脂酶与“融合剂”及化学相关“非融合剂”的多晶型相行为进行了比较研究。我们证明，只有融合剂能诱导膜脂从双层向六方（HII）相发生构象变化。（2）红细胞（空壳）膜形成非双层结构的倾向与不同单油酸甘油酯膜浓度下红细胞的融合程度相关。在单油酸甘油酯的某个临界膜浓度以上，空壳膜形成HII相的倾向增加，同时完整红细胞中的融合过程也显著增加。（3）这些观察结果与促融合脂质的共同作用机制一致，即诱导形成诸如长圆柱体（HII构型）或反相胶束等倒置脂质结构，这些结构在融合事件中起中间体作用。
