Wojcieszyn J W, Schlegel R A, Lumley-Sapanski K, Jacobson K A
J Cell Biol. 1983 Jan;96(1):151-9. doi: 10.1083/jcb.96.1.151.
The mechanism by which polyethylene glycol (PEG) mediates cell fusion has been studied by examining the movements of membrane lipids and proteins, as well as cytoplasmic markers, from erythrocytes to monolayers of cultured cells to which they have been fused. Fluorescence and freeze-fracture electron microscopy and fluorescence recovery after photobleaching have yielded the following results: (a) In the presence of both fusogenic and nonfusogenic PEG membranes are brought together at closely apposed contact regions. (b) Fluorescent lipid probes quickly spread from the membranes of erythrocytes to cultured cells in the presence of both fusogenic and nonfusogenic PEG. (c) Proteins of the erythrocyte membranes were never observed to diffuse into the cultured cell membrane. (d) Water-soluble proteins did not diffuse from the erythrocyte interior into the target cell cytoplasm until the PEG was removed. These data suggest that the coordinate action of two distinct components is necessary for fusion as mediated by PEG. Presumably, the polymer itself promotes close apposition of the adjacent cell membranes but the fusion stimulus is provided by the additives contained in commercial PEG.
通过检测膜脂和膜蛋白的运动以及细胞质标记物从红细胞到与其融合的培养细胞单层的运动，研究了聚乙二醇（PEG）介导细胞融合的机制。荧光显微镜、冷冻断裂电子显微镜以及光漂白后的荧光恢复实验得到了以下结果：（a）在促融合和非促融合PEG存在的情况下，膜在紧密相邻的接触区域聚集在一起。（b）在促融合和非促融合PEG存在的情况下，荧光脂质探针迅速从红细胞膜扩散到培养细胞。（c）从未观察到红细胞膜蛋白扩散到培养细胞膜中。（d）直到PEG被去除，水溶性蛋白才从红细胞内部扩散到靶细胞细胞质中。这些数据表明，PEG介导的融合需要两个不同成分的协同作用。推测聚合物本身促进相邻细胞膜的紧密贴合，但融合刺激是由商业PEG中含有的添加剂提供的。
