Stokke B T, Elgsaeter A
Biochim Biophys Acta. 1981 Feb 6;640(3):640-5. doi: 10.1016/0005-2736(81)90094-8.
Employing viscometry, human spectrin heterodimers and heterotetramers were analyzed in aqueous solution containing different amounts of salt. (1) In aqueous 0.1 M NaCl, pH 7.5, at 4 degrees C, the intrinsic viscosity of isolated human spectrin heterodimers and heterotetramers was found to be 40 +/- 6 and 79 +/- 7 ml/g, respectively. (2) The intrinsic viscosity of isolated human spectrin heterodimers and heterotetramers increased to 78 +/- 8 and 180 +/- 10 ml/g, respectively, as the ionic strength of the solution was reduced to about 2 mM. (3) This viscometric study indicates that isolated human spectrin heterodimers and heterotetramers are flexible molecules with a contour length of at least 110 and 200 nm, respectively.
利用粘度测定法，在含有不同盐量的水溶液中对人血影蛋白异二聚体和异四聚体进行了分析。（1）在4℃、pH 7.5的0.1 M NaCl水溶液中，分离得到的人血影蛋白异二聚体和异四聚体的特性粘度分别为40±6和79±7 ml/g。（2）当溶液的离子强度降低至约2 mM时，分离得到的人血影蛋白异二聚体和异四聚体的特性粘度分别增加至78±8和180±10 ml/g。（3）这项粘度测定研究表明，分离得到的人血影蛋白异二聚体和异四聚体是柔性分子，其轮廓长度分别至少为110和200 nm。
