[Some features of nuclear genome transcription at early stages of liver regeneration. Effect of cytoplasmic factors on the RNA-polymerase activity of isolated nuclei].

Cytosol from the regenerating liver significantly activates the RNA-polymerase activity of isolated nuclei in normal liver. This activation occurs immediately after partial hepatectomy, reaches its maximum after the 1st post-operative hour and is practically eliminated after 1,5 hrs. The effect is thermolabile and is completely blocked by a preliminary administration of cycloheximide. Fractionation of cytosol on DEAE-cellulose reveals significant chromatographic differences of the activating factor as compared to that described in literature, which is present both in normal and regenerating liver. An important role of this factor in reprogramming of the genome from the hepatocytes at the preproliferation stage is postulated.