Ermolaev V I, Baranov O K
Biokhimiia. 1980 Jul;45(7):1208-14.
A procedure for isolation of a very high density Lpm-lipoprotein (Lpm) consisting of 7-15% polyethyleneglycol precipitation and subsequent gel-filtration on a bio-gel A-5m column was developed. An immunoelectrophoretically pure Lpm with molecular weight of about 800 000 was obtained. The procedure used does not change the allo- and heteroantigenic properties of Lpm.
开发了一种分离极高密度Lpm脂蛋白(Lpm)的方法,该方法包括7%-15%的聚乙二醇沉淀以及随后在生物凝胶A-5m柱上进行凝胶过滤。获得了一种免疫电泳纯的Lpm,其分子量约为800000。所采用的方法不会改变Lpm的同种和异种抗原特性。