Tedesco F, Kahane I, Zanella A, Giovanetti A M, Sirchia G
Blood. 1981 May;57(5):900-5.
Lipids extracted with chloroform-methanol from red blood cell membranes of 7 PNH and 13 control subjects were used for the preparation of liposomes, which were then examined with the reactive lysis test. PNH liposomes lysed to a higher extent than control liposomes as indicated by the higher dilution of the limiting complement reagent that was necessary to lyse 50% of the PNH liposomes. A similar finding was also observed with liposomes made of lipids from AET-treated red cells. The enhanced reactive lysis can be attributed to the polar lipid fraction, as indicated by the increased lysis of hybrid liposomes prepared from this polar lipids extracted from PNH erythrocyte membrane and lipids extracted from normal erythrocyte membrane. The increased susceptibility to reactive lysis does not seem to be specific of PNH liposomes, since it was also observed with liposomes prepared from lipids of red cells from beta-thalassemia major and autoimmune hemolytic disease.
用氯仿 - 甲醇从7名阵发性睡眠性血红蛋白尿（PNH）患者和13名对照受试者的红细胞膜中提取脂质，用于制备脂质体，然后用反应性溶解试验进行检测。PNH脂质体的溶解程度高于对照脂质体，这表现为溶解50%的PNH脂质体所需的极限补体试剂的稀释度更高。在用经AET处理的红细胞的脂质制成的脂质体中也观察到了类似的结果。反应性溶解增强可归因于极性脂质部分，这由从PNH红细胞膜提取的极性脂质和从正常红细胞膜提取的脂质制备的杂合脂质体的溶解增加所表明。对反应性溶解敏感性增加似乎并非PNH脂质体所特有，因为在由重型β地中海贫血和自身免疫性溶血性疾病患者的红细胞脂质制备的脂质体中也观察到了这一现象。
