Batten J R, Newman D L
Cardiovasc Res. 1980 Oct;14(10):590-600. doi: 10.1093/cvr/14.10.590.
The uptake of 131I-albumin by the wall of the isolated pig thoracic aorta was investigated in vitro to achieve good control of the experimental conditions. The lumena of the arteries were perfused with a balanced salt solution (Hanks') containing the radioactively labelled albumin. Albumin flux was studied at three static intralumenal pressures, viz 6.7, 13.3 and 20 kPa (50, 100 and 150 mmHg) and uptake (normalised per unit dry tissue weight) was found to be approximately doubled on increasing the pressure from 6.7 to 20 kPa (50 to 150 mmHg). With a mean pressure of 6.7 kPa (50 mmHg) the uptake was studied in the presence of sinusoidal pressure oscillations of amplitude in the range 1.3 to 6.0 kPa (10 to 45 mmHg) at frequencies 1, 5 and 11 Hz. At each frequency the uptake was found to be dependent on the pressure amplitude. The dependence at 5 Hz was significantly stronger than at 1 Hz (P < 0.05) and although not significant there was a suggestion that the uptake was higher at 5 than 11 Hz. There was no significance and no suggestion of a difference at 1 and 11 Hz. The possible influence on uptake of the associated oscillatory wall shear component has been considered. The relevance of these findings to macromolecular transport and to the characteristic localisation of atheroma within the arterial tree is discussed.
为了更好地控制实验条件，在体外研究了131I标记白蛋白在离体猪胸主动脉壁的摄取情况。动脉管腔用含有放射性标记白蛋白的平衡盐溶液（汉克斯液）进行灌注。在三种静态管腔内压力下，即6.7、13.3和20 kPa（50、100和150 mmHg）研究白蛋白通量，发现当压力从6.7 kPa（50 mmHg）增加到20 kPa（150 mmHg）时，摄取量（以单位干组织重量归一化）大约增加了一倍。在平均压力为6.7 kPa（50 mmHg）的情况下，研究了在频率为1、5和11 Hz时，幅度在1.3至6.0 kPa（10至45 mmHg）范围内的正弦压力振荡存在时的摄取情况。在每个频率下，摄取量均取决于压力幅度。5 Hz时的依赖性明显强于1 Hz（P < 0.05），虽然不显著，但有迹象表明5 Hz时的摄取量高于11 Hz。1 Hz和11 Hz时没有显著性差异，也没有差异的迹象。已经考虑了相关振荡壁面剪切分量对摄取的可能影响。讨论了这些发现与大分子运输以及动脉树内动脉粥样硬化特征定位的相关性。
