Belmin J, Corman B, Merval R, Tedgui A
Institut National de la Santé et de la Recherche Médicale, Unité 141, Hôpital Lariboisière, Paris, France.
Am J Physiol. 1993 Mar;264(3 Pt 2):H679-85. doi: 10.1152/ajpheart.1993.264.3.H679.
Age-related changes in macromolecular transport across the arterial wall were investigated in 10-, 20-, and 30-mo-old WAG/Rij rats. Animals were injected with 125I- and 131I-labeled albumin, 90 and 5 min before they were killed, respectively. The transmural distribution of relative concentration of tracers in the aortic wall was obtained using en face serial sectioning technique. The apparent endothelial permeability to albumin calculated from the distribution of 5-min 131I-labeled albumin concentrations was significantly enhanced in 20- and 30-mo-old rats compared with 10-mo-old rats. The apparent distribution volume of albumin within the media, estimated as the mean medial 125I-labeled albumin concentration, was not significantly changed in 20-mo-old rats but was significantly decreased in the 30-mo-old animals. These age-related changes in the macromolecular transport suggest that the entry of plasma macromolecules in the aged arterial wall might be enhanced, whereas the efflux through the media may be impeded, possibly contributing to their trapping in the subendothelium.
在10月龄、20月龄和30月龄的WAG/Rij大鼠中研究了动脉壁大分子转运的年龄相关变化。分别在处死动物前90分钟和5分钟给动物注射125I和131I标记的白蛋白。采用表面连续切片技术获得示踪剂在主动脉壁中相对浓度的跨壁分布。根据5分钟时131I标记白蛋白浓度的分布计算得出的白蛋白表观内皮通透性，在20月龄和30月龄大鼠中比10月龄大鼠显著增强。根据平均中层125I标记白蛋白浓度估算的白蛋白在中膜内的表观分布容积，在20月龄大鼠中无显著变化，但在30月龄动物中显著降低。大分子转运的这些年龄相关变化表明，老年动脉壁中血浆大分子的进入可能增强，而通过中膜的流出可能受阻，这可能导致它们滞留在内皮下。
