Feuvray D, Plouët J
Circ Res. 1981 May;48(5):740-7. doi: 10.1161/01.res.48.5.740.
We studied mitochondrial structure and intermediates of fatty acid metabolism in mitochondria isolated from ischemic hearts. By electron microscopy, no structural difference was detected between mitochondria isolated from control hearts and from ischemic hearts receiving glucose as the only substrate. However, major differences were observed in mitochondria obtained from control and ischemic hearts receiving both glucose and palmitate. These hearts contained a higher portion of damaged mitochondria. However, measurements of marker enzyme activities failed to show that more mitochondria were lost during the isolation procedure in ischemic than in control hearts. Many densely staining areas (or amorphous densities) were observed in the isolated mitochondria of ischemic hearts. These amorphous densities have an appearance similar to that observed in the intact ischemic heart under the same perfusion conditions. Levels of long-chain acyl-CoA in mitochondria isolated from hearts receiving glucose alone were practically the same for control and ischemic hearts and were only slightly increased in mitochondria of ischemic hearts receiving both glucose and palmitate. On the other hand, levels of long-chain acyl carnitine in mitochondria of ischemic hearts were twice those found in control hearts. The mitochondrial level of long-chain acyl carnitine was approximately four times higher in the ischemic hearts receiving palmitate compared to those receiving no palmitate. This rise in long-chain acyl carnitine levels in mitochondria isolated fom ischemic hearts receiving palmitate may be related to modifications of the mitochondrial structure and to the appearance of amorphous densities.
我们研究了从缺血心脏分离出的线粒体的结构以及脂肪酸代谢中间体。通过电子显微镜观察，在以葡萄糖作为唯一底物的情况下，从对照心脏和缺血心脏分离出的线粒体之间未检测到结构差异。然而，在同时接受葡萄糖和棕榈酸酯的对照心脏和缺血心脏所获得的线粒体中观察到了主要差异。这些心脏中受损线粒体的比例更高。然而，标记酶活性的测量结果未能表明，与对照心脏相比，缺血心脏在分离过程中线粒体丢失得更多。在缺血心脏分离出的线粒体中观察到许多深染区域（或无定形致密物）。这些无定形致密物的外观与在相同灌注条件下完整缺血心脏中观察到的相似。仅接受葡萄糖的心脏分离出的线粒体中，对照心脏和缺血心脏的长链酰基辅酶A水平实际上相同，而在同时接受葡萄糖和棕榈酸酯的缺血心脏线粒体中仅略有增加。另一方面，缺血心脏线粒体中的长链酰基肉碱水平是对照心脏中的两倍。与未接受棕榈酸酯的缺血心脏相比，接受棕榈酸酯的缺血心脏线粒体中的长链酰基肉碱水平大约高四倍。从接受棕榈酸酯的缺血心脏分离出的线粒体中长链酰基肉碱水平的升高可能与线粒体结构的改变以及无定形致密物的出现有关。
