Feuvray D
Adv Myocardiol. 1983;4:441-8. doi: 10.1007/978-1-4757-4441-5_40.
A study correlating functional, metabolic, and ultrastructural changes in the ischemic myocardium was conducted on isolated working rat hearts, both in the presence and absence of fatty acid. Glucose alone (11 mM) or glucose plus palmitic acid (1.5 mM) were used as metabolic substrates. A 60-min period of whole-heart ischemia resulted in a more dramatic morphological alteration in those hearts receiving palmitate than in those receiving no palmitate. In ischemic hearts receiving palmitate, intramitochondrial amorphous densities of both rounded and elongated types were observed. These densities did not develop in hearts receiving glucose alone over the same period of ischemia. Such morphological alterations were associated with a more severe deterioration of mechanical function in the presence of palmitate. Biochemical determinations of fatty acid derivatives showed increased tissue levels of acyl esters of CoA and carnitine in ischemic hearts, but levels of long-chain acyl carnitine were much higher in those ischemic hearts receiving palmitate. Furthermore, from the data obtained on isolated mitochondria, it appeared that the mitochondrial level of long-chain acyl carnitine was approximately four times higher in the ischemic hearts receiving palmitate than in those receiving no palmitate. This great rise in mitochondrial levels of long-chain acyl carnitine correlated with modifications of the mitochondrial structure and with the appearance of amorphous densities.
在有脂肪酸和无脂肪酸的情况下，对离体工作的大鼠心脏进行了一项关于缺血心肌功能、代谢和超微结构变化相关性的研究。单独使用葡萄糖（11 mM）或葡萄糖加棕榈酸（1.5 mM）作为代谢底物。60分钟的全心缺血导致接受棕榈酸的心脏比未接受棕榈酸的心脏出现更显著的形态学改变。在接受棕榈酸的缺血心脏中，观察到线粒体内有圆形和细长形的无定形致密物。在相同缺血时间内，单独接受葡萄糖的心脏未出现这些致密物。这种形态学改变与棕榈酸存在时机械功能更严重的恶化有关。脂肪酸衍生物的生化测定显示，缺血心脏中辅酶A和肉碱的酰基酯组织水平升高，但接受棕榈酸的缺血心脏中长链酰基肉碱水平更高。此外，从离体线粒体获得的数据来看，接受棕榈酸的缺血心脏中长链酰基肉碱的线粒体水平似乎比未接受棕榈酸的心脏高约四倍。长链酰基肉碱线粒体水平的大幅升高与线粒体结构的改变以及无定形致密物的出现相关。
