Bertani-Dziedzic L M, Krstulovic A M, Dziedzic S W, Gitlow S E, Cerqueira S
Clin Chim Acta. 1981 Feb 19;110(1):1-8. doi: 10.1016/0009-8981(81)90293-x.
A sensitive and specific direct analysis of urinary normetanephrine (NMN) and metanephrine (MN) was achieved utilizing reversed-phase high performance liquid chromatography and electrochemical detection. Individual specimens from "control" subjects and those with pheochromocytoma were hydrolyzed and the metanephrines separated from other urinary constituents by elution with ammonia from a Dowex CG-50 resin. Chromatographic peaks were identified by retention behavior, co-chromatography with reference compounds, ratio of responses at various oxidation potentials and stopped-flow UV spectra of the collected fractions. The NMN and MN content for the control subjects was between 0.086 and 0.21 (mean - 0.14) microgram/mg creatinine and 0.012 and 0.092 (mean = 0.039) microgram/mg creatinine, respectively. The values for subjects with pheochromocytoma varied from 1.5 to 27.5 (mean = 9.9) microgram/mg creatinine for NMN and 0.10 to 1.60 (mean = 0.86) microgram/mg creatine for MN. The patient with ganglioneuroma had an NMN of 4.1 and an MN of 0.80 microgram/mg creatinine. While this method permits discrimination between those patients with pheochromocytoma and the overwhelming majority of hypertensive patients, it may ultimately be further extended to separate normal subjects from those with more subtle derangements in catecholamine metabolism.
采用反相高效液相色谱法和电化学检测法，实现了对尿中去甲变肾上腺素（NMN）和变肾上腺素（MN）的灵敏且特异的直接分析。对“对照”受试者和嗜铬细胞瘤患者的个体样本进行水解，并用氨从Dowex CG - 50树脂上洗脱，将变肾上腺素与其他尿液成分分离。通过保留行为、与参考化合物的共色谱分析、不同氧化电位下的响应比以及收集馏分的停流紫外光谱来鉴定色谱峰。对照受试者的NMN和MN含量分别在0.086至0.21（平均值 = 0.14）微克/毫克肌酐和0.012至0.092（平均值 = 0.039）微克/毫克肌酐之间。嗜铬细胞瘤患者的NMN值在1.5至27.5（平均值 = 9.9）微克/毫克肌酐之间，MN值在0.10至1.60（平均值 = 0.86）微克/毫克肌酐之间。神经节神经瘤患者的NMN为4.1微克/毫克肌酐，MN为0.80微克/毫克肌酐。虽然该方法能够区分嗜铬细胞瘤患者和绝大多数高血压患者，但最终可能会进一步扩展，以区分正常受试者和儿茶酚胺代谢有更细微紊乱的患者。
