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包被活性炭在雌激素受体活性测定中的应用。

The use of coated charcoal in the determination of oestrogen receptor activity.

作者信息

Ralet P G, Brombacher P J

出版信息

Eur J Nucl Med. 1981 Apr;6(4):159-62. doi: 10.1007/BF00253166.

DOI:10.1007/BF00253166
PMID:7215381
Abstract

Oestrogen receptor activity in mammary tumour and healthy tissues were determined by using a method of incubation with coated charcoal. It appears that both increased charcoal concentration and prolonged incubation leads to erroneous results, which are probably due to loss of receptor protein. It is concluded that receptor activity assays must be performed under strictly standardized experimental conditions.

摘要

通过用包被活性炭孵育法测定乳腺肿瘤组织和健康组织中的雌激素受体活性。结果表明,活性炭浓度增加和孵育时间延长都会导致错误结果,这可能是由于受体蛋白的丢失所致。得出的结论是,受体活性测定必须在严格标准化的实验条件下进行。

相似文献

1
The use of coated charcoal in the determination of oestrogen receptor activity.包被活性炭在雌激素受体活性测定中的应用。
Eur J Nucl Med. 1981 Apr;6(4):159-62. doi: 10.1007/BF00253166.
2
Quantitation of oestrogen receptors: use of solid-phase antisteroid antibodies to quantify binding sites and determination of dissociation constant.雌激素受体的定量:使用固相抗类固醇抗体定量结合位点并测定解离常数。
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Quantitative aspects of the E2 receptor assay for human breast tumour cytosol using dextran-coated charcoal.使用葡聚糖包被活性炭对人乳腺肿瘤胞质溶胶进行 E2 受体测定的定量方面。
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Isoelectric focusing of oestradiol receptor protein from human mammary carcinoma--a comparison with dextran coated charcoal assay.人乳腺癌雌二醇受体蛋白的等电聚焦——与葡聚糖包被活性炭分析法的比较
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Histological detection of oestrogen receptor in human breast carcinomas.
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引用本文的文献

1
Steroid receptors in breast cancer: sources of inter-laboratory variation in dextran-charcoal assays.
Breast Cancer Res Treat. 1987;9(3):175-89. doi: 10.1007/BF01806378.

本文引用的文献

1
Coated charcoal immunoassay of insulin.胰岛素的包被炭免疫测定法。
J Clin Endocrinol Metab. 1965 Oct;25(10):1375-84. doi: 10.1210/jcem-25-10-1375.
2
Radioimmunoassay and saturation analysis. Separation techniques in saturation analysis.放射免疫测定与饱和分析。饱和分析中的分离技术。
Br Med Bull. 1974 Jan;30(1):32-7. doi: 10.1093/oxfordjournals.bmb.a071164.
3
Standards for the assessment of estrogen receptors in human breast cancer. Report of a workshop on September 29, 1972, at the Antoni van Leeuwenhoek-Huis, Amsterdam.
Eur J Cancer (1965). 1973 May;9(5):379-81. doi: 10.1016/0014-2964(73)90055-8.