Aronson J F, Cristofalo V J
In Vitro. 1981 Jan;17(1):61-70. doi: 10.1007/BF02618032.
Cells obtained by tryptic digestion of the surface of intact adult and fetal Fischer 344 rat lungs were plated on glass fragments. Epithelial cell lines were readily established by selecting fragments with 2 to 10 cells 2 days after plating and growing them in F12 K media containing 10% fetal bovine serum (FBS). These cell lines and new lines that can be easily obtained a provide a reliable source of diploid, density-inhibited epithelial cells. These cells of mesothelial origin may serve as models for the study of mesothelial cells in situ.
通过胰蛋白酶消化完整的成年和胎儿Fischer 344大鼠肺表面获得的细胞接种于玻璃碎片上。接种2天后，通过挑选含有2至10个细胞的碎片并在含有10%胎牛血清（FBS）的F12 K培养基中培养，上皮细胞系很容易建立。这些细胞系以及能够轻易获得的新细胞系提供了可靠的二倍体、密度抑制上皮细胞来源。这些间皮来源的细胞可作为原位研究间皮细胞的模型。
