Mueller S N, Rosen E M, Levine E M
Science. 1980 Feb 22;207(4433):889-91. doi: 10.1126/science.7355268.
The life-span in vitro and other proliferative characteristics of a strain of endothelial cells cloned from the aorta of a fetal calf were examined. Cultures of these cells had a replicative life-span of approximately 80 cumulative population doublings. Growth rates in the logarithmic phase and plateau densities decreased as the cumulative population-doubling level increased. After approximately 65 percent of the life-span of a culture was completed, the percentage of cells that incorporated [3H]thymidine during a 24-hour labeling period began to decrease rapidly. The cells expressed factor VIII antigen and their intercellular borders were stainable with silver nitrate throughout the life-span of each culture. Average cellular attachment size increased more than threefold between cumulative population-doubling levels 41 and 80. The facility with which cloned strains of endothelial cells can be isolated should encourage further exploitation of this important cell culture model.
对从胎牛主动脉克隆的一株内皮细胞的体外寿命及其他增殖特性进行了检测。这些细胞培养物的复制寿命约为80次累积群体倍增。随着累积群体倍增水平的增加，对数期的生长速率和平板密度降低。在培养物寿命约65%完成后，在24小时标记期内掺入[3H]胸腺嘧啶核苷的细胞百分比开始迅速下降。这些细胞表达因子VIII抗原，并且在每个培养物的整个寿命期间其细胞间边界可用硝酸银染色。在累积群体倍增水平41至80之间，平均细胞附着大小增加了三倍多。内皮细胞克隆株的分离便利性应会促使对这一重要细胞培养模型进行进一步开发。
