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大鼠壁层胸膜间皮细胞的培养方法。

Culture procedure of mesothelial cells from the rat parietal pleura.

作者信息

Thiollet J, Jaurand M C, Kaplan H, Bignon J, Hollande E

出版信息

Biomedicine. 1978 Apr;29(2):69-73.

PMID:667291
Abstract

Cultures were made of mesothelial cells obtained by scraping the parietal pleura of the adult rats. The growth was restricted to close polyhedric epithelial-like cells, forming a monolayer. The cellular proliferation continued until the 7th day, followed by a stationary phases. In subcultures the mesothelial cells kept their epithelial type. The cultures were stopped on the 20th day.

摘要

培养物由刮取成年大鼠壁层胸膜获得的间皮细胞制成。生长仅限于紧密的多面体上皮样细胞,形成单层。细胞增殖持续到第7天,随后进入静止期。在传代培养中,间皮细胞保持其上皮类型。培养在第20天停止。

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Culture procedure of mesothelial cells from the rat parietal pleura.大鼠壁层胸膜间皮细胞的培养方法。
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