Sebille B, Thuaud N, Tillement J P
J Chromatogr. 1981 Jan 16;204:285-91. doi: 10.1016/s0021-9673(00)81670-2.
The binding to human serum albumin of some drugs (warfarin, furosemide and phenylbutazone) has been studied by high-performance liquid chromatography. Two methods have been used and compared, based on the measurement of the ligand retention volume under different conditions. The obtained total affinities of the drugs for the protein are in accordance with our previous results. The equilibrium saturation method leads easily to ni and Ki parameters from the retention volume of the ligand.
已通过高效液相色谱法研究了某些药物（华法林、呋塞米和保泰松）与人血清白蛋白的结合情况。基于在不同条件下配体保留体积的测量，使用并比较了两种方法。所获得的药物与蛋白质的总亲和力与我们之前的结果一致。平衡饱和法可根据配体的保留体积轻松得出ni和Ki参数。
