Guinebault P R, Broquaire M, Braithwaite R A
J Chromatogr. 1981 Jan 16;204:329-33. doi: 10.1016/s0021-9673(00)81675-1.
A high-performance liquid chromatographic method for the measurement of nifuroxazide in plasma is described. The technique is based on the single extraction of the drug from buffered plasma with chloroform, using nifuratel as internal standard. The chromatographic system consisted of a 15 cm x 4.6 mm I.D. stainless-steel column packed with Spherisorb ODS, 5 micrometer, and the mobile phase was acetonitrile-orthophosphoric acid (pH 2.5) (30:70). The method was able to measure accurately plasma nifuroxazide concentrations down to 2 ng . ml-1 using 2 ml of sample with no interference from endogenous compounds. The coefficients of variation of the method at 200 and 2 ng . ml-1 were 3% and 15%, respectively, and the calibration graph was linear in this range. The use of automatic injection makes the method suitable for the routine analysis of large numbers of samples.
本文描述了一种用于测定血浆中硝呋太尔的高效液相色谱法。该技术基于用氯仿从缓冲血浆中单次提取药物，以硝呋太尔作为内标。色谱系统由一根15 cm×4.6 mm内径、填充5微米Spherisorb ODS的不锈钢柱组成，流动相为乙腈 - 正磷酸（pH 2.5）（30:70）。该方法使用2 ml样品能够准确测定低至2 ng·ml⁻¹的血浆硝呋太尔浓度，且不受内源性化合物干扰。该方法在200 ng·ml⁻¹和2 ng·ml⁻¹时的变异系数分别为3%和15%，在此范围内校准曲线呈线性。自动进样的使用使该方法适用于大量样品的常规分析。
