Dubruc C, Caqueret H, Bianchetti G
J Chromatogr. 1981 Jan 16;204:335-9. doi: 10.1016/s0021-9673(00)81676-3.
A specific and sensitive high-performance liquid chromatographic method for the quantitative determination of vincamine at therapeutic concentrations in plasma is described. The column was packed with Spherisorb ODS 5 micrometer, and the mobile phase was acetonitrile-potassium phosphate (0.02 M, pH 2.3) (50:50) with a flow-rate of 10 ml/min. Detection was at 230 nm. Using automated large-volume injection of a non-eluting solvent (0.02 M potassium phosphate) the method was capable of the analysis of a large number of samples daily. The coefficient of variation of the procedure was 4.8% at a plasma vincamine concentration of 10 ng/ml
描述了一种用于定量测定血浆中治疗浓度长春胺的特异性灵敏高效液相色谱法。色谱柱填充有5微米的Spherisorb ODS，流动相为乙腈 - 磷酸钾（0.02 M，pH 2.3）（50:50），流速为10 ml/min。检测波长为230 nm。使用非洗脱溶剂（0.02 M磷酸钾）的自动大体积进样，该方法能够每天分析大量样品。在血浆长春胺浓度为10 ng/ml时，该方法的变异系数为4.8%
