Jindal S P, Lutz T, Cooper T B
J Chromatogr. 1980 Dec 12;221(2):301-8. doi: 10.1016/s0378-4347(00)84315-7.
A quantitative gas chromatographic-mass spectrometric assay was developed for the determination of phenelzine in human plasma. Phenelzine, in aqueous solution or in plasma reacts at room temperature with pentafluorobenzaldehyde to form quantitatively a hydrazone derivative. The derivative has good gas chromatographic characteristics. The assay utilizes selected ion monitoring in a gas chromatographic effluent, the molecular ion generated by electron impact ionization of phenelzine derivative. Phenelzine-d, was synthesized and used as an internal standard. The assay can measure 2 ng/ml of the drug with about 10% precision. The method was used for the determination of steady state levels of phenelzine in the plasma of patients taking a therapeutic dose of the drug.
已开发出一种定量气相色谱 - 质谱分析法，用于测定人血浆中的苯乙肼。苯乙肼在水溶液或血浆中于室温下与五氟苯甲醛反应，定量生成腙衍生物。该衍生物具有良好的气相色谱特性。该分析方法利用气相色谱流出物中的选择离子监测，即苯乙肼衍生物经电子轰击电离产生的分子离子。合成了氘代苯乙肼并用作内标。该分析方法能够以约10%的精密度测定2 ng/ml的药物。该方法用于测定服用治疗剂量该药物的患者血浆中苯乙肼的稳态水平。
