一种用于补体激活的荧光测定法。
A fluorescent assay for complement activation.
作者信息
Caporale L H, Gaber S S, Kell W, Götze O
出版信息
J Immunol. 1981 May;126(5):1963-5.
PMID:7217674
Abstract
We report here a rapid assay for the complement enzymes CVFBb, C4b2a and Cls. This assay involves the use of a peptide substrate that releases a fluorescent coumarin derivative (AMC) upon cleavage by the convertase. The substrate, BocLeuGlyArgAMC, was chosen because its sequence is similar to the carboxyl terminus of C3a, and identical to that of C5a. The Km of this substrate are about 125 microM for the C3/5 convertase CVFBb, 169 microM for C4b2a, and 140 microM for C1s.
摘要
我们在此报告一种针对补体酶CVFBb、C4b2a和Cls的快速检测方法。该检测方法使用一种肽底物，该底物在被转化酶切割后会释放出一种荧光香豆素衍生物（AMC）。选择底物BocLeuGlyArgAMC是因为其序列与C3a的羧基末端相似，且与C5a的序列相同。该底物对于C3/5转化酶CVFBb的米氏常数约为125微摩尔，对于C4b2a为169微摩尔，对于C1s为140微摩尔。
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