一种用于评估硫胺素营养充足性的改良红细胞转酮醇酶测定法的评估。

An evaluation of a modified erythrocyte transketolase assay for assessing thiamine nutritional adequacy.

作者信息

Boni L, Kieckens L, Hendrikx A

出版信息

J Nutr Sci Vitaminol (Tokyo). 1980;26(5):507-14. doi: 10.3177/jnsv.26.507.

DOI:10.3177/jnsv.26.507

Abstract

Transketolase (TK) activity was assessed by incubating hemolyzed whole blood with ribose-5-phosphate both in the presence (TKsat.) and in the absence (TK) of thiamine pyrophosphate (TPP). The amount of pentoses utilized between 5 and 60 min after the start of the incubation was taken as a measure of the TK activity. The TPP effect was expressed at (TKsat. - TK)/TK x 100. The coefficient of variation for the TPP effect determination was 9.6% within the TPP effect range from 6 to 22%. Within the TPP effect range from 24 to 46%, the coefficient of variation was 8.7%. The mean TPP effect as measured in a group of healthy individuals amounted to 13.5%, with a range from 8.3 to 18.5%. In a group of obese women submitted to an energy-restricted diet, the TPP effect ranged from 12.7 to 30%. In patients suffering from Wernicke's encephalopathy, the TPP effect varied from 28 to 67%.

摘要

通过在存在（转酮醇酶饱和状态，TKsat.）和不存在（转酮醇酶，TK）硫胺素焦磷酸（TPP）的情况下，将溶血全血与5-磷酸核糖一起孵育来评估转酮醇酶（TK）活性。将孵育开始后5至60分钟内利用的戊糖量作为TK活性的度量。TPP效应表示为（TKsat. - TK）/TK×100。在TPP效应范围为6%至22%时，TPP效应测定的变异系数为9.6%。在TPP效应范围为24%至46%时，变异系数为8.7%。一组健康个体中测得的平均TPP效应为13.5%，范围为8.3%至18.5%。在一组接受能量限制饮食的肥胖女性中，TPP效应范围为12.7%至30%。在患有韦尼克脑病的患者中，TPP效应在28%至67%之间变化。