Wallace J E, Shimek E L, Harris S C
J Anal Toxicol. 1981 Jan-Feb;5(1):20-3. doi: 10.1093/jat/5.1.20.
A method is described for the determination of amitriptyline, nortriptyline, imipramine and desipramine at low nanogram concentrations in serum. Separation and quantitation are performed by reversed-phase, isocratic, high-performance liquid chromatography utilizing ultraviolet detection at 210 nm. An internal standard, chloramitriptyline, is used as an aid to quantitation. Recovery of all four tricyclics is greater than 84%. The relative standard deviation for the analysis of amitriptyline, nortriptyline, imipramine and desipramine is less than 6% at a concentration of 100 ng/mL.
本文描述了一种测定血清中低纳克浓度的阿米替林、去甲替林、丙咪嗪和地昔帕明的方法。采用反相等度高效液相色谱法，于210nm波长处进行紫外检测，实现分离和定量分析。使用内标氯米帕明辅助定量。所有四种三环类药物的回收率均大于84%。在浓度为100ng/mL时，阿米替林、去甲替林、丙咪嗪和地昔帕明分析的相对标准偏差小于6%。
