Segatti M P, Nisi G, Grossi F, Mangiarotti M, Lucarelli C
Laboratorio di Analisi Chimico Cliniche, Ospedale Infantile Burlo Garofolo, Trieste, Italy.
J Chromatogr. 1991 Jan 4;536(1-2):319-25. doi: 10.1016/s0021-9673(01)89265-7.
An isocratic reversed-phase high-performance liquid chromatographic procedure is presented for the simultaneous detection of desipramine, nortriptyline, imipramine, amitriptyline and clomipramine in serum. Drugs are extracted after sample alkalinization and separated from each other on an octyl reversed-phase with n-butylamine as mobile phase modifier. Detection is achieved at 254 nm. The recovery of tricyclic antidepressants (92-110%) has good precision, with a relative standard deviation of less than 5%. Being rapid and simple, the method is suitable for the emergency clinical laboratory.
本文介绍了一种等度反相高效液相色谱法，用于同时检测血清中的地昔帕明、去甲替林、丙咪嗪、阿米替林和氯米帕明。样品碱化后提取药物，并在以正丁胺为流动相改性剂的辛基反相柱上彼此分离。在254nm处进行检测。三环类抗抑郁药的回收率为92%-110%，精密度良好，相对标准偏差小于5%。该方法快速简便，适用于临床急诊实验室。
