Konishi E, Hotta S
Microbiol Immunol. 1980;24(9):847-59. doi: 10.1111/j.1348-0421.1980.tb02889.x.
The mechanism of the action of tannic acid (TA) Chikungunya virus (CHIKV) was investigated. Both infectivity and hemagglutination (HA) activity of CHIKV were reduced by treatment with TA in vitro. Aggregation of the TA-treated virus particles was observed by electron microscopy. The reaction was reversible, depending on the pH of the mixture. However, mere dilution of the TA-virus mixture or addition of other protein, such as bovine serum albumin, did not restore the lost infectivity. TA also suppressed the infectivity of RNA extracted from the virus and the HA activity of the viral membrane. The affinity between TA and CHIKV structural proteins (E1, E2, and C) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a discontinuous buffer system and the order of affinity was found to be C greater than E1 greater than E2. Specific conditions for binding of TA to each of the virus proteins were investigated.
研究了单宁酸（TA）对基孔肯雅病毒（CHIKV）的作用机制。在体外，用TA处理可降低CHIKV的感染性和血凝（HA）活性。通过电子显微镜观察到经TA处理的病毒颗粒发生聚集。该反应是可逆的，取决于混合物的pH值。然而，单纯稀释TA-病毒混合物或添加其他蛋白质（如牛血清白蛋白）并不能恢复丧失的感染性。TA还抑制了从病毒中提取的RNA的感染性以及病毒膜的HA活性。通过在不连续缓冲系统中进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定了TA与CHIKV结构蛋白（E1、E2和C）之间的亲和力，发现亲和力顺序为C＞E1＞E2。研究了TA与每种病毒蛋白结合的特定条件。
