Konishi E, Hotta S
Microbiol Immunol. 1979;23(7):659-67. doi: 10.1111/j.1348-0421.1979.tb00507.x.
The present report describes not only the effects of tannic acid (TA; belonging to hydrolyzable tannins) and its related compounds upon the infectivity of Chikungunya virus (CHIKV) but also the mechanism involved in this phenomenon. Our data show that TA inactivates CHIKV in vitro. Since the inactivating effect turned out to be pH-dependent and was suppressed by bovine serum albumin, it is most probable that the virus-inactivating capacity of TA is attributable to its preferential binding to proteins of virus particles. Examination on the virus-inactivating capacities of some TA-related compounds and comparison of their structures indicated that the active site of TA and its analogues might be the phenolic hydroxyl groups in their molecules. It seems that the active groups interact with the proteins of virus particles, resulting in a reduction or loss of viral infectivity. Discussion is made on the specificity of the actions of tannins and the possibility of application thereof to chemicals which are useful to investigate the nature and properties of viral proteins.
本报告不仅描述了鞣酸（TA；属于可水解单宁）及其相关化合物对基孔肯雅病毒（CHIKV）感染性的影响，还阐述了这一现象背后的机制。我们的数据表明，TA在体外可使CHIKV失活。由于这种失活效应呈pH依赖性且受到牛血清白蛋白的抑制，因此TA的病毒失活能力很可能归因于其与病毒颗粒蛋白质的优先结合。对一些与TA相关的化合物的病毒失活能力进行检测并比较其结构后发现，TA及其类似物的活性位点可能是其分子中的酚羟基。似乎这些活性基团与病毒颗粒的蛋白质相互作用，导致病毒感染性降低或丧失。文中还讨论了单宁作用的特异性以及将其应用于有助于研究病毒蛋白质性质的化学物质的可能性。
