Gerber G B, Maes J
Radiat Environ Biophys. 1980;18(4):249-56. doi: 10.1007/BF01324267.
The number and type of stem cells in spleen and bone marrow of mice were determined after exposure to a single dose of 150 R on day 6, to a single dose of 500 R on day 6 or day 9 or to a fractionated dose of 150 R + 350 R on day 6 and 9. The stem cells were assayed on the basis of colony forming units (CFU) in spleen and of incorporation of iododeoxyuridine in spleen and bone marrow of lethally irradiated host mice. During the first months of life, the number of stem cells in non-irradiated mice increases markedly in bone marrow and slightly in spleen. Irradiation causes a long-lasting depression in stem cells, particularly in bone marrow and affecting preferentially erythropoietic precursor cells. Following a dose of only 150 R, the number of CFU in bone marrow is still below control levels 24 days later. An exposure to 500 R fractionated between day 6 and 9 has a markedly greater effect on stem cells in the spleen than the same dose given in a single application either at day 6 or 9.
在第6天给予小鼠单次150伦琴剂量照射、在第6天或第9天给予单次500伦琴剂量照射，或在第6天和第9天给予分次剂量150伦琴 + 350伦琴照射后，测定小鼠脾脏和骨髓中干细胞的数量和类型。基于致死性照射宿主小鼠脾脏中的集落形成单位（CFU）以及脾脏和骨髓中碘脱氧尿苷的掺入情况来检测干细胞。在生命的最初几个月，未照射小鼠骨髓中的干细胞数量显著增加，脾脏中的干细胞数量略有增加。照射会导致干细胞长期减少，尤其是在骨髓中，并且优先影响红系前体细胞。仅给予150伦琴剂量后，24天后骨髓中的CFU数量仍低于对照水平。在第6天和第9天分次给予500伦琴剂量对脾脏中的干细胞的影响明显大于在第6天或第9天单次给予相同剂量。
