Eremin O, Ashby J, Plumb D
J Immunol Methods. 1978;24(3-4):257-67. doi: 10.1016/0022-1759(78)90130-8.
Deionised water is an efficient red blood cell lytic agent with no demonstrable deleterious effects on ADCC and natural cytotoxicity (blood and tonsil). Treatment with isotonic ammonium chloride, however, produces a significant reduction of both ADCC and natural cytotoxicity. This reduction is less pronounced if the treatment is carried out at 4 degrees C and is temporary, recovery occurring over a 24 h period of incubation at 37 degrees C.
去离子水是一种有效的红细胞裂解剂，对抗体依赖的细胞介导的细胞毒性作用（ADCC）和自然细胞毒性（血液和扁桃体）没有明显的有害影响。然而，用等渗氯化铵处理会使ADCC和自然细胞毒性都显著降低。如果在4℃进行处理，这种降低不太明显，并且是暂时的，在37℃孵育24小时后会恢复。
