Savary C A, Phillips J H, Lotzová E
J Immunol Methods. 1979;25(2):189-92. doi: 10.1016/0022-1759(79)90055-3.
In the present study the effect of ammonium chloride on murine natural killer (NK) cell-mediated cytotoxicity to T cell lymphoma, YAC-1 was studied. It was found that ammonium chloride treatment significantly reduced the cytotoxicity of splenic NK cells without any detectable change in cell viability. It is, therefore, suggested to avoid ammonium chloride treatment in order to obtain the realistic reflection of murine NK cell activities.
在本研究中，研究了氯化铵对小鼠自然杀伤（NK）细胞介导的针对T细胞淋巴瘤YAC-1的细胞毒性的影响。发现氯化铵处理显著降低了脾NK细胞的细胞毒性，而细胞活力没有任何可检测到的变化。因此，建议避免氯化铵处理，以便获得小鼠NK细胞活性的真实反映。
