叙利亚仓鼠的溶血补体及其成分：对未感染和感染彭亨布鲁线虫的五个品系的研究

Hemolytic complement and its components in Syrian hamsters: a study of five strains uninfected and infected with Brugia pahangi.

作者信息

Barta O, Oyekan P P, Malone J B, Klei T R

出版信息

Adv Exp Med Biol. 1981;134:103-10. doi: 10.1007/978-1-4757-0495-2_10.

Abstract

Complement profiles were tested in outbred (LVG) Syrian hamsters (Mesocricetus auratus) and compared to the MHA, LHC, PD4, and CB inbred strains. The total C and C component concentrations in the sera varied among the strains and were in the following ranges in untreated animals (in CH50 units per ml): total C, 140-260 (undetectable in PD4 and CB); Cl, 14,000-25,000; C2, 200-800 (except PD 4 and CB); C3, 40,000 and 80,000; C4, 2,000-2,800 (except PD4 and CB); C4, 40,000-80,000; C6, 3,600-6,000 (undetectable in PD4 and CB); C7, 50,000-350,000; C8, 10,000-30,000; C9, 30,000-60,000. The PD4 and CB strains had undetectable total C and C6, and their exact C2 and C4 levels could not be determined, but were lower than in the other strains. The MHA strain had the highest total C levels, but had significantly lower (1/3 or less) C7 levels than the other strains of hamsters. Infection of hamsters with the filarid nematode Brugia pahangi for four to five months produced moderate decreases in the total C and C3 levels, but varied changes in other C components. Six infected and three uninfected animals died during the experiment from spontaneous enteritis and weight loss.

摘要

对远交（LVG）叙利亚仓鼠（金仓鼠）的补体谱进行了检测，并与MHA、LHC、PD4和CB近交系进行比较。各品系血清中的总补体（C）和补体成分浓度有所不同，在未处理动物中，其范围如下（以每毫升CH50单位计）：总补体，140 - 260（在PD4和CB中未检测到）；C1，14,000 - 25,000；C2，200 - 800（PD4和CB除外）；C3，40,000至80,000；C4，2,000 - 2,800（PD4和CB除外）；C4，40,000 - 80,000；C6，3,600 - 6,000（在PD4和CB中未检测到）；C7，50,000 - 350,000；C8，10,000 - 30,000；C9，30,000 - 60,000。PD4和CB品系的总补体和C6未检测到，其确切的C2和C4水平无法确定，但低于其他品系。MHA品系的总补体水平最高，但C7水平比其他仓鼠品系显著低（三分之一或更低）。用丝状线虫彭亨布鲁线虫感染仓鼠四至五个月后，总补体和C3水平适度下降，但其他补体成分变化各异。实验期间，六只感染动物和三只未感染动物因自发性肠炎和体重减轻死亡。