Pérez-Clavier R, Harrison R G
J Pathol. 1978 Apr;124(4):219-25. doi: 10.1002/path.1711240406.
Forty-two mature albino rats were subjected to the ligation of selected lymphatic vessels close to, and at a distance from, the testis. At periods from 3 days to 1 year both testes were studied at their equatorial level in order to determine histological changes. The operated testes showed a mild degree of damage 3 days after operation and moderate damage at 7 days, while damage of a severe degree increased to a maximum over a postoperative period of 90 days. One-hundred-and-eighty days after operation a few tubules presented the typical appearance of spermatogenic destruction. After 1 year recovery is virtually complete. Changes in the contralateral testis indicate an immune response to the damage induced on the ipsilateral side by the experimental interruption of selected lymph vessels, where interstitial oedema was confined sectorially to the medial half of the testis. In the contralateral testis, oedema was predictably diffuse throughout; the distribution of tubular deformities and the degree of cellular damage were less well marked and 1 year after operation the testis had almost completely recovered. Tubular deformities and cellular changes in the germinal epithelium are primarily dependent on interference with the lymph flow and are not secondary to ischaemia.
42只成年白化大鼠的特定淋巴管在靠近睾丸及远离睾丸处被结扎。在术后3天至1年的不同时间段，对双侧睾丸的赤道平面进行研究，以确定组织学变化。手术侧睾丸在术后3天显示轻度损伤，7天显示中度损伤，而重度损伤在术后90天内达到最大值。术后180天，少数曲细精管呈现出生精破坏的典型表现。1年后，恢复基本完成。对侧睾丸的变化表明其对同侧因选择性淋巴管实验性阻断所诱导的损伤产生了免疫反应，此时间质水肿呈扇形局限于睾丸内侧半区。在对侧睾丸中，水肿则可预见地弥漫分布；曲细精管畸形的分布及细胞损伤程度不太明显，术后1年睾丸几乎完全恢复。生精上皮的曲细精管畸形和细胞变化主要取决于淋巴流的干扰，而非继发于缺血。
