Sugiyama K, Amano Y
Arch Virol. 1981;67(3):241-51. doi: 10.1007/BF01318134.
Biological and morphological properties of a virus, isolated from the intestine of infant mice with clinical signs of diarrhea and designated as diarrhea virus of infant mice (DVIM), were examined. The first infective virus was detected on the cells 4 hours post infection, followed by rapid release of the virus into the culture fluids. Virus-induced syncytia in BALB/c-3T3 cell cultures caused hemadsorption at 4 degrees C and viral antigens were shown to be located in the cytoplasm of the syncytia by immunofluorescent techniques. By scanning electron-microscopy, budding virus-like particles were detected on the surface of virus-induced syncytia. Morphologically the virus was shown to be enveloped and approximately 100 nm in diameter. Two types of projections were demonstrated, one type of projection was club-shaped, 20 nm in length and the other type was small, granular, 5 nm in length. The latter type of projection might be the basal part of the club-shaped type and related to the hemagglutinating activity.
从出现腹泻临床症状的幼鼠肠道中分离出一种病毒，命名为幼鼠腹泻病毒（DVIM），并对其生物学和形态学特性进行了检测。感染后4小时在细胞上检测到首批感染性病毒，随后病毒迅速释放到培养液中。在BALB/c - 3T3细胞培养物中，病毒诱导形成的多核巨细胞在4℃时引起血细胞吸附，通过免疫荧光技术显示病毒抗原位于多核巨细胞的细胞质中。通过扫描电子显微镜观察，在病毒诱导的多核巨细胞表面检测到出芽的病毒样颗粒。从形态学上看，该病毒有包膜，直径约100nm。显示有两种类型的突起，一种突起呈棒状，长20nm，另一种较小，呈颗粒状，长5nm。后一种类型的突起可能是棒状突起的基部，与血凝活性有关。
