Dello C P, Belpaire F M, Kint J A, Fraeyman N H
Heymans Institute of Pharmacology, Medical School, Univeristy of Ghent, Belgium.
J Pharmacol Methods. 1987 Dec;18(4):335-45. doi: 10.1016/0160-5402(87)90065-9.
Dog alpha-1-acid glycoprotein was purified to homogeneity from dog serum in a three-step procedure involving precipitation with sulphosalicylic acid, isoelectric focusing and size exclusion chromatography. The molecular heterogeneity in the peptide part and in the carbohydrate part of the molecule was investigated with analytical isoelectric focusing in a narrow pH range and crossed immunoaffinity electrophoresis with concanavalin A (con A) in the first-dimension gel. Up to seven molecular forms with different isoelectric points were found, whereas only a single con A-dependent molecular form was detected.
犬α-1-酸性糖蛋白通过三步法从犬血清中纯化至同质，这三步包括用磺基水杨酸沉淀、等电聚焦和尺寸排阻色谱。在狭窄pH范围内进行分析性等电聚焦以及在一维凝胶中用伴刀豆球蛋白A（Con A）进行交叉免疫亲和电泳，研究了该分子肽段部分和碳水化合物部分的分子异质性。发现了多达七种具有不同等电点的分子形式，而仅检测到一种依赖Con A的分子形式。
