Pollack S, Campana T
Biochim Biophys Acta. 1981 Apr 3;673(4):366-73. doi: 10.1016/0304-4165(81)90468-2.
Hemolysates, prepared from guinea pig reticulocytes incubated with 59Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an Mx 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The 59Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.
用59Fe标记的血清孵育豚鼠网织红细胞制备的溶血产物，通过分子筛色谱可分离为五个峰：铁蛋白、转铁蛋白、血红蛋白、分子量为17000的Mx组分和低分子量组分。通过血红素提取和等电聚焦证明，血红蛋白峰还含有一种非血红蛋白成分（III-X）。转铁蛋白、III-X组分和低分子量组分是网织红细胞孵育过程中最先积累放射性铁的。这些组分中的59Fe也会发生追踪。因此，提示这些组分在铁掺入血红素过程中作为前体发挥作用。
