Weaver J, Zhan H, Pollack S
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
Biochem J. 1990 Jan 15;265(2):415-9. doi: 10.1042/bj2650415.
Recent work has provided new evidence that ATP is the major constituent of the low-Mr iron pool in the reticulocyte. The interaction of the iron complex of ATP with mitochondria was investigated in the present experiments. When ATP-Fe3+ was incubated with mitochondria, Fe3+, free of ATP, bound with high affinity to Fe3+ receptors on the mitochondria. The binding was saturable and reversible. Iron which was complexed to PPi, nitrilotriacetate, citrate, ADP and GTP also showed saturable binding to mitochondria; Fe3+ complexed to AMP bound non-specifically, as did Fe2+/ascorbate complexed to AMP bound non-specifically, as did Fe2+/ascorbate and Fe2+/dithionite.
近期的研究工作提供了新的证据,表明ATP是网织红细胞中低分子量铁池的主要成分。在本实验中,对ATP的铁复合物与线粒体的相互作用进行了研究。当ATP-Fe3+与线粒体一起孵育时,游离于ATP的Fe3+以高亲和力与线粒体上的Fe3+受体结合。这种结合是可饱和且可逆的。与PPi、次氮基三乙酸、柠檬酸、ADP和GTP络合的铁也显示出与线粒体的可饱和结合;与AMP络合的Fe3+非特异性结合,与Fe2+/抗坏血酸和Fe2+/连二亚硫酸盐一样,与AMP络合的Fe2+/抗坏血酸也非特异性结合。