Martin A, Biol M C, Arrambide E, Richard M, Louisot P
Biochimie. 1981 Mar;63(3):241-5. doi: 10.1016/s0300-9084(81)80198-8.
The soluble fucosyl-transferase of the rat small intestinal mucosa was purified by passing through a Sephadex G-100 column, then resolved in two isoenzymes F1 (pHi = 4,47) and F2 (pHi = 4,96) by isoelectric focusing. The use in the first step of DEAE-cellulose instead of Sephadex G-100 leads to another component F3 (pHi = 8,70). Kinetic parameters (KM et Vm) for the three isoenzymes are given. The enzymatic mechanism seems to be a bi-bi random type for the three isoenzymes, and F3 appears as the most active species.
大鼠小肠黏膜的可溶性岩藻糖基转移酶经Sephadex G - 100柱纯化，然后通过等电聚焦分离为两种同工酶F1（等电点 = 4.47）和F2（等电点 = 4.96）。第一步使用DEAE - 纤维素而非Sephadex G - 100会产生另一种组分F3（等电点 = 8.70）。给出了这三种同工酶的动力学参数（米氏常数KM和最大反应速度Vm）。这三种同工酶的酶促机制似乎都是双底物随机类型，并且F3表现为活性最强的种类。
