[Biosynthesis of glycoconjugates in the arterial intima. VII. Isolation and purification of fucosyl-transferase from the aorta].
作者信息
Serres-Guillaumond M, Louisot P
出版信息
Paroi Arterielle. 1979 Jul;5(2):87-95.
PMID:514638
Abstract
A fucosyl-transferase, catalysing the transfer of fucose, from GDP-fucose to proteinic or polyprenic acceptors, exists in microsomes of intima-media from aorta. Solubilization and purification of this enzyme is obtained by treatment of microsomes with Cemulsol NPT 12, column chromatography on DEAE-cellulose, Dowex 1 X 8 and electrofocusing. Only one molecular species is detected at pHi = 4.7.
摘要
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