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[Biosynthesis of glycoconjugates in the arterial intima. VII. Isolation and purification of fucosyl-transferase from the aorta].

作者信息

Serres-Guillaumond M, Louisot P

出版信息

Paroi Arterielle. 1979 Jul;5(2):87-95.

PMID:514638
Abstract

A fucosyl-transferase, catalysing the transfer of fucose, from GDP-fucose to proteinic or polyprenic acceptors, exists in microsomes of intima-media from aorta. Solubilization and purification of this enzyme is obtained by treatment of microsomes with Cemulsol NPT 12, column chromatography on DEAE-cellulose, Dowex 1 X 8 and electrofocusing. Only one molecular species is detected at pHi = 4.7.

摘要

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