Kirby L T, Applegarth D A, Davidson A G, Wong L T, Hardwick D F
Clin Chem. 1981 May;27(5):678-8.
We assayed more than 5000 blood spots dried on filter paper and approximately 1000 serum samples for immunoreactive trypsin, with commercial reagents (Behring and Sorin). The assay procedures were modified so that newborn screening is technically feasible. Both kits are satisfactory for serum assay, but the Sorin materials are better adapted for blood spot analysis. Immunoreactive trypsin in blood spots rapidly decreases with specimen age, but is stable in frozen serum. Values for premature infants do not differ significantly from those for full-term babies. Children with cystic fibrosis were readily distinguished from those without, up to at least one year of age.
我们使用商业试剂（贝林和索林）对5000多个滤纸上干燥的血斑以及约1000份血清样本进行了免疫反应性胰蛋白酶检测。对检测程序进行了修改，以便新生儿筛查在技术上可行。两种试剂盒用于血清检测都令人满意，但索林的材料更适合血斑分析。血斑中的免疫反应性胰蛋白酶会随着样本存放时间迅速降低，但在冷冻血清中则稳定。早产儿的值与足月儿的值没有显著差异。患有囊性纤维化的儿童与未患病的儿童很容易区分开来，至少在一岁之前是这样。
