Keyser J W, Fifield R, Watkins G L
Clin Chem. 1981 May;27(5):736-8.
When we compared four commercially available preparations of human serum albumin with the human serum standard IFCC 74/1 by radial immunodiffusion, by immunoprecipitation turbidimetry, by laser nephelometry, and by "rocket" immunoelectrophoresis, three of the preparations gave almost "theoretical" results with the immunoprecipitation turbidimetric method. Results by the other three methods tended inconsistently to be low. Four pools of normal human serum were also analyzed for albumin by these four immunochemical methods, again with IFCC 74/1 as the reference standard. The results were virtually identical with those obtained by fractionation with 1.8 mol/L sodium sulfate and determination of protein nitrogen in the filtrate. We suggest that a combination of (a) fractionation of a pool of normal human serum in this way and (b) critical comparison with selected commercial preparations of human serum albumin will permit standardization of the serum albumin determination.
当我们通过放射免疫扩散法、免疫沉淀比浊法、激光散射比浊法以及“火箭”免疫电泳法，将四种市售人血清白蛋白制剂与国际临床化学联合会（IFCC）74/1人血清标准品进行比较时，其中三种制剂采用免疫沉淀比浊法得出了几乎“理论”上的结果。而采用其他三种方法得出的结果往往不一致且偏低。另外，还使用这四种免疫化学方法，以IFCC 74/1作为参考标准，对四组正常人血清样本进行了白蛋白分析。其结果与通过用1.8 mol/L硫酸钠分级分离并测定滤液中蛋白质氮所得到的结果几乎完全相同。我们认为，将（a）以这种方式对正常人血清样本进行分级分离，与（b）与选定的市售人血清白蛋白制剂进行严格比较相结合，将能够实现血清白蛋白测定的标准化。
