Bouzek J, Mancal P
Institute of Sera and Vaccines, Prague, Czechoslovakia.
J Hyg Epidemiol Microbiol Immunol. 1989;33(1):105-11.
Quantitation of IgG, IgA and IgM immunoglobulins and C3, C4 components in human serum samples by the radial immunodiffusion technique and by the nephelometric and turbidimetric assays was compared using the linear regression analysis method. Comparisons of the two methods run in polyethylene glycol showed close agreement between methods and a relatively high degree of correlation between the parameters studied. Compared to the radial immunodiffusion technique, nephelometry and turbidimetry gave good correlation between parameters, but the agreement between tests was worse, especially in the case of C3 component determinations in fresh samples of patients' sera. All tests were carried out using the Q-antisera and Control human serum preparations SEVAC.
采用线性回归分析方法，比较了用放射免疫扩散技术以及比浊法和散射比浊法对人血清样本中的IgG、IgA和IgM免疫球蛋白以及C3、C4成分进行定量的情况。在聚乙二醇中进行的两种方法的比较显示，方法之间一致性良好，所研究参数之间具有较高的相关性。与放射免疫扩散技术相比，比浊法和散射比浊法在参数之间具有良好的相关性，但检测之间的一致性较差，尤其是在患者血清新鲜样本中C3成分测定的情况下。所有检测均使用Q抗血清和SEVAC人血清对照制剂进行。
