Carter J
J Reprod Fertil. 1978 Nov;54(2):433-9. doi: 10.1530/jrf.0.0540433.
The expression of paternal and maternal antigens on three types of mouse trophoblast cultured in vitro has been examined with the mixed haemadsorption test. Primary trophoblastic giant cells outgrown from blastocysts and placental trophoblast from 16-day pregnancies expressed antigens of both sources at all stages in culture. Secondary trophoblastic giant cells outgrown from the tip of 8-day ectoplacental cones did not give a positive reaction until they had outgrown for 3-5 days. Microdensitometry after Feulgen staining showed that the cells which gave a positive mixed haemadsorption response were polyploid. Incubation of blastocysts and ectoplacental cone tissue with progesterone, oestradiol-17beta and hCG did not affect the results.
通过混合血细胞吸附试验检测了体外培养的三种小鼠滋养层细胞上父源和母源抗原的表达。从囊胚生长出的原代滋养层巨细胞以及来自妊娠16天胎盘的滋养层细胞在培养的各个阶段都表达了两种来源的抗原。从8天胎盘外锥体尖端生长出的二代滋养层巨细胞在生长3 - 5天之前没有给出阳性反应。福尔根染色后的显微密度测定表明，给出阳性混合血细胞吸附反应的细胞是多倍体。用孕酮、雌二醇 - 17β和人绒毛膜促性腺激素孵育囊胚和胎盘外锥体组织并不影响结果。
