Drake B L, Rodger J C
Immunology. 1985 Jun;55(2):325-9.
Primary cultures of murine trophoblast (ectoplacental cone and mid-term placenta) and their supernatants were found to inhibit in vitro lymphocyte proliferative responses to concanavalin A (77-87%) and allo-antigen (52-84%). However, cultures and cell-conditioned media from non-trophoblastic tissues (embryonic sac, adult lung and liver, and B16 melanoma line) produced similar results. In all cases, the inhibitory effects were not due to reduced cell viability. Addition of anti-progesterone serum to the ectoplacental cone-lymphocyte co-cultures, at a concentration known to bind the available trophoblast-derived progesterone, did not overcome the observed suppression. The results clearly demonstrate that a range of cultured cell types, and their conditioned media, will suppress immune responses in vitro. We conclude that cultured trophoblast is not an appropriate model for studies of placental immunoregulation.
已发现小鼠滋养层（外胎盘锥和中期胎盘）的原代培养物及其上清液可在体外抑制淋巴细胞对伴刀豆球蛋白A（77 - 87%）和同种异体抗原（52 - 84%）的增殖反应。然而，来自非滋养层组织（胚囊、成年肺和肝脏以及B16黑色素瘤细胞系）的培养物和细胞条件培养基也产生了类似结果。在所有情况下，抑制作用并非由于细胞活力降低。将抗孕酮血清添加到外胎盘锥 - 淋巴细胞共培养物中（浓度已知可结合可获得的滋养层来源的孕酮），并不能克服所观察到的抑制作用。结果清楚地表明，一系列培养的细胞类型及其条件培养基在体外会抑制免疫反应。我们得出结论，培养的滋养层不是研究胎盘免疫调节的合适模型。
