Van Vlasselaer P, Vandeputte M
Cell Immunol. 1984 Feb;83(2):422-32. doi: 10.1016/0008-8749(84)90322-8.
The modification of the immunological response by murine trophoblast cells of different sources was investigated using the mixed lymphocyte reaction (MLR) and the cell mediated lympholysis (CML) test. MLR between C57BL (H-2b) stimulator splenocytes (mitomycin C treated in the unidirectional MLR) and BALB/c (H-2d) responder lymph node cells were markedly suppressed by trophoblast of ectoplacental cone (EPC) and placental origin. The same in vitro effect was observed with supernatants (SN) of trophoblast cells and with supernatants of blastocysts. Addition of anti-progesterone serum (APS), anti-testosterone serum (RAT), and anti-immunoglobulin serum (RAHIg) in serial dilutions to the trophoblast-MLR system revealed that the immunosuppressive effect of trophoblast giant cells and trophoblast giant cell culture supernatants can be abolished with APS. Identical results were obtained with APS added to immunosuppressive doses of progesterone. CML between C57BL responder lymph node cells and mitomycin C-treated BALB/c stimulator spleen cells was also markedly suppressed when trophoblast of EPC origin was added. A similar suppression of cytotoxic T-cell induction was seen when progesterone was added to the system. The immunosuppressive action of trophoblast as detected in vitro is likely to play an important role in the maintainance of pregnancy by protecting the semiallogeneic conceptus against immune aggression by the maternal immune system.
利用混合淋巴细胞反应（MLR）和细胞介导的淋巴细胞溶解（CML）试验，研究了不同来源的小鼠滋养层细胞对免疫反应的调节作用。在单向MLR中经丝裂霉素C处理的C57BL（H-2b）刺激脾细胞与BALB/c（H-2d）反应性淋巴结细胞之间的MLR，受到外胎盘锥（EPC）和胎盘来源的滋养层细胞的显著抑制。滋养层细胞的上清液（SN）和囊胚的上清液也观察到了相同的体外效应。将系列稀释的抗孕酮血清（APS）、抗睾酮血清（RAT）和抗免疫球蛋白血清（RAHIg）添加到滋养层-MLR系统中发现，APS可以消除滋养层巨细胞和滋养层巨细胞培养上清液的免疫抑制作用。将APS添加到免疫抑制剂量的孕酮中也获得了相同的结果。当添加EPC来源的滋养层细胞时，C57BL反应性淋巴结细胞与经丝裂霉素C处理的BALB/c刺激脾细胞之间的CML也受到显著抑制。当向系统中添加孕酮时，也观察到了对细胞毒性T细胞诱导的类似抑制作用。体外检测到的滋养层细胞的免疫抑制作用可能在维持妊娠中发挥重要作用，通过保护半同种异体的孕体免受母体免疫系统的免疫攻击。
