Turriff D E, Lim R
Dev Neurosci. 1981;4(2):110-7. doi: 10.1159/000112746.
Glia maturation factor (GMF) is a protein first isolated from the adult pig brain. GMF-like activity can be demonstrated in rat organs, including brain, kidney and heart. The activity in these organs is low in newborn animals, but increases with development, reaching the adult level in 1 or 2 weeks. GMF-like activities in the various organs are similar in physicochemical properties, being heat-labile, susceptible to proteolytic enzymes, and are associated with an acidic molecule of large size. Cultured rat glioblasts and C6 glioma cells, but not their conditioned media, contain large amounts of endogenous GMF-like activity. GMF obtained from brains and cultured glial cells also possess mitogenic action. Subcellular fractionation localizes GMF-like activity in the cytosol and in microsomal and nerve ending fractions. GMF-like activity is also detectable in bovine, sheep, monkey and human brains. The results suggest that GMF is ubiquitous in distribution, and at least a portion of it may be associated with the structural components of the cells.
胶质细胞成熟因子（GMF）是一种最初从成年猪脑中分离出来的蛋白质。在大鼠的器官中，包括脑、肾和心脏，均可显示出类似GMF的活性。这些器官中的活性在新生动物中较低，但随着发育而增加，在1或2周内达到成年水平。各器官中类似GMF的活性在物理化学性质上相似，不耐热，易受蛋白水解酶影响，且与一种大分子酸性分子相关。培养的大鼠成胶质细胞和C6胶质瘤细胞含有大量内源性类似GMF的活性，但其条件培养基中则没有。从脑中获得的GMF和培养的神经胶质细胞也具有促有丝分裂作用。亚细胞分级分离将类似GMF的活性定位于细胞质、微粒体和神经末梢部分。在牛、羊、猴和人的脑中也可检测到类似GMF的活性。结果表明，GMF分布广泛，且至少有一部分可能与细胞的结构成分相关。
