Kato T, Yamakawa Y, Lim R, Turriff D E, Tanaka R
Neurochem Res. 1981 Apr;6(4):401-12. doi: 10.1007/BF00963855.
Optimal bioassay conditions for bovine glia maturation factor (GMF) were determined among glial cells from normal glioblasts to glioma cells. Rat glioblasts 4-8 days after subculture show the highest response t GMF with regard to morphological transformation and mitogenic activity. Bovine GMF enhances DNA synthesis of rat glioblasts at 12 h after stimulation; maximum incorporation of [methyl-3H]thymidine was detected at 18 h. GMF increases twofold the saturation density of rat glioblasts but does not alter that of C6 astrocytoma cells. The apparent inhibition of mitogenic activity of high doses of GMF is seen in both normal and malignant glial cells.
在从正常成胶质细胞到神经胶质瘤细胞的神经胶质细胞中确定了牛神经胶质成熟因子（GMF）的最佳生物测定条件。传代培养4 - 8天后的大鼠成胶质细胞对GMF在形态转化和促有丝分裂活性方面表现出最高反应。牛GMF在刺激后12小时增强大鼠成胶质细胞的DNA合成；在18小时检测到[甲基-3H]胸腺嘧啶核苷的最大掺入量。GMF使大鼠成胶质细胞的饱和密度增加两倍，但不改变C6星形细胞瘤细胞的饱和密度。在正常和恶性神经胶质细胞中均可见高剂量GMF对促有丝分裂活性的明显抑制。
