Stenhouse F O, Williams K L
Differentiation. 1981;18(1):1-9. doi: 10.1111/j.1432-0436.1981.tb01099.x.
The cell patterning (proportion of spores, stalk cells, and basal disk cells) of individual asexual fruiting bodies of haploid and isogenic diploid strains of D. discoideum was examined to test the hypothesis that the patterning mechanism is based on the 'sensing' of only a single parameter, e.g., cell volume, in dividing the aggregate into the three cell types. If cell patterning is based on sensing only a single parameter, there is no reason to predict a change in cell patterning with ploidy change, and thus haploid and isogenic diploid strains should not differ in their cell patterning. The cell patterning of each of the three pairs of haploid and isogenic diploid strains examined was different. Therefore we conclude that the cell patterning mechanism must involve at least two components not changing in the same way with change in ploidy. The cell patterning of both the haploid and the diploid strains was qualitatively similar, i.e., relationships between the three cell types were described by equations of the same form in the haploid and diploid strains. However a quantitative change in cell patterning led to an increased percentage of stalk and basal disk cells in each diploid compared to its parent haploid. The ratio of basal disk to stalk cell was also greater in the diploids than in their parent haploids. We conclude that these are general ploidy-related changes because the cell patterning of each of the three parent haploid strains was different; the average percentage of stalk cells was 11.6% for X22 (12.4% for its diploid DU162), 20.5% for NP73 (27.2% for its diploid DP62), and 24.5% for HU127 (29.1% for its diploid DU310). One possible patterning mechanism could involve a diffusible signal (s), which shows gene dosage, interacting with cell-surface molecules which we predict occupy a limited number of sites per unit area of the cell membrane. The observed change in cell patterning leading to an increased percentage of stalk cells in diploid strains is predicted from such a model involving diffusible signal interacting with cell-surface molecules.
对盘基网柄菌单倍体和同基因二倍体菌株的单个无性繁殖子实体的细胞模式（孢子、柄细胞和基盘细胞的比例）进行了研究，以检验以下假设：在将聚集体分为三种细胞类型时，模式形成机制仅基于对单个参数（例如细胞体积）的“感知”。如果细胞模式仅基于对单个参数的感知，那么就没有理由预测细胞模式会随着倍性变化而改变，因此单倍体和同基因二倍体菌株在细胞模式上不应存在差异。所检测的三对单倍体和同基因二倍体菌株中，每一对的细胞模式都不同。因此，我们得出结论，细胞模式形成机制必须至少涉及两个随着倍性变化而不以相同方式变化的成分。单倍体和二倍体菌株的细胞模式在定性上相似，即单倍体和二倍体菌株中三种细胞类型之间的关系都由相同形式的方程描述。然而，细胞模式的定量变化导致每个二倍体中柄细胞和基盘细胞的百分比相对于其亲本单倍体有所增加。二倍体中基盘细胞与柄细胞的比例也高于其亲本单倍体。我们得出结论，这些是与倍性相关的一般变化，因为三种亲本单倍体菌株中每一种的细胞模式都不同；X22的柄细胞平均百分比为11.6%（其二倍体DU162为12.4%），NP73为20.5%（其二倍体DP62为27.2%），HU127为24.5%（其二倍体DU310为29.1%）。一种可能的模式形成机制可能涉及一种可扩散信号，该信号显示基因剂量，并与细胞表面分子相互作用，我们预测这些细胞表面分子在细胞膜单位面积上占据有限数量的位点。从这种涉及可扩散信号与细胞表面分子相互作用的模型可以预测，观察到的细胞模式变化会导致二倍体菌株中柄细胞百分比增加。
