Sannohe Y, Hiratsuka R
Gastroenterol Jpn. 1981;16(1):25-32. doi: 10.1007/BF02820421.
Cytofluorometric analysis of nuclear DNA content was performed in carcinoma cells of the stomach and the esophagus and the exclusive four types of the DNA histogram patterns were established. In 33 patients with carcinoma of the stomach 7 (87.5%) out of 8 with Type I and 3 (50%) out of 6 with Type II in the DNA histogram pattern were the superficial types of carcinomas. Eighteen (81.8%) out of 22 patients with advanced gastric carcinoma belonged to Type III or IV in the DNA histogram pattern and there was only one of the superficial spreading carcinoma seen in these Types. The superficial spreading carcinoma (Stout 10) is generally reported to take more favorable clinical course than the usual advanced ones and the above data were supported that the DNA histogram pattern was associated with prognostic important property of invasive growth. In 34 patients with carcinoma of the esophagus 4 out of 7 with Type I in the DNA histogram pattern have been living more than 3 years following surgery with poor lymphatic permeation (14.3%) around the tumor. While, 6 out of 9 with Type IV had an early recurrence of cancer causing postoperative death within a year after surgery with high incidence of lymphatic permeation (77.8%) around the tumor. Thus, the DNA histogram pattern in the cytofluorometric DNA analysis of the tumor cells seems to give valuable information about the malignancy degree of the individual tumor, which is not possible to obtain only from the cytomorphological features.
对胃癌和食管癌的癌细胞进行了细胞核DNA含量的细胞荧光分析，并建立了四种独特的DNA直方图模式。在33例胃癌患者中，DNA直方图模式为I型的8例中有7例（87.5%），II型的6例中有3例（50%）为浅表型癌。22例进展期胃癌患者中，18例（81.8%）的DNA直方图模式属于III型或IV型，这些类型中仅见1例浅表扩散型癌。一般报道浅表扩散型癌（Stout 10）的临床病程比通常的进展期癌更有利，上述数据支持DNA直方图模式与浸润性生长的预后重要特性相关。在34例食管癌患者中，DNA直方图模式为I型的7例中有4例术后存活超过3年，肿瘤周围淋巴渗透较差（14.3%）。而IV型的9例中有6例癌症早期复发，术后一年内死亡，肿瘤周围淋巴渗透发生率高（77.8%）。因此，肿瘤细胞的细胞荧光DNA分析中的DNA直方图模式似乎能提供有关单个肿瘤恶性程度的有价值信息，而这仅从细胞形态学特征是无法获得的。
