Mirovský P, Valkoun A
Folia Parasitol (Praha). 1981;28(1):23-7.
Simple methods for the preparation of purified suspensions of T. gondii zoites from mouse peritoneal exudate and HeLa tissue cultures are described. The suspensions from the mouse peritoneal exudate were prepared by mechanical disintegration of host cells and tryptic digestion. The average yield of purified Toxoplasma was 78%, with average purity of 99.96%. The suspensions from the tissue cultures were prepared by differential centrifugation and tryptic digestion. The average yield of Toxoplasma was 67% with average purity of 99.67%. An advantage of these procedures is their feasibility.
本文描述了从小鼠腹腔渗出液和HeLa组织培养物中制备纯化的刚地弓形虫速殖子悬液的简单方法。从小鼠腹腔渗出液中制备悬液是通过机械破碎宿主细胞和胰蛋白酶消化来实现的。纯化的弓形虫平均产量为78%，平均纯度为99.96%。从组织培养物中制备悬液是通过差速离心和胰蛋白酶消化来完成的。弓形虫的平均产量为67%，平均纯度为99.67%。这些方法的一个优点是其可行性。
