Dempster R P
Exp Parasitol. 1984 Apr;57(2):195-207. doi: 10.1016/0014-4894(84)90080-8.
Many methods have been proposed for removing contaminating host cells from mouse peritoneal exudates infected with Toxoplasma gondii tachyzoites. Of these, eight established methods were compared. They were density gradients, sonication and trypsin digestion, differential centrifugation, haemolysin digestion, filtration through glass wool and cellulose columns, and sintered glass and polycarbonate filtration. The methods were assessed for zoite recovery, host cell removal, effect on zoite viability and antigenic integrity, time, cost, and ease. They were almost all capable of removing greater than 90% of the mouse leucocytes, but in some cases this resulted in low zoite recoveries. The sonication and trypsin method produced the best zoite recovery and highest purity, but appeared to affect zoite viability and antigenic integrity. The haemolysin digestion procedure has been adopted by our laboratory because of its high recovery of zoites, and it is inexpensive, quick, and easy to perform.
已经提出了许多方法来从感染刚地弓形虫速殖子的小鼠腹腔渗出物中去除污染的宿主细胞。在这些方法中，对八种既定方法进行了比较。它们是密度梯度法、超声处理和胰蛋白酶消化法、差速离心法、溶血素消化法、通过玻璃棉和纤维素柱过滤法，以及烧结玻璃和聚碳酸酯过滤法。对这些方法进行了虫体回收率、宿主细胞去除率、对虫体活力和抗原完整性的影响、时间、成本和操作简便性等方面的评估。它们几乎都能够去除超过90%的小鼠白细胞，但在某些情况下，这会导致虫体回收率较低。超声处理和胰蛋白酶法产生了最佳的虫体回收率和最高的纯度，但似乎会影响虫体活力和抗原完整性。溶血素消化程序因其高虫体回收率而被我们实验室采用，而且它成本低、速度快且易于操作。
